Journal: bioRxiv
Article Title: Discovery of a pentose as a cytosine nucleobase modification in Shewanella phage Thanatos-1 genomic DNA mediating enhanced resistance towards host restriction systems
doi: 10.1101/2024.02.27.582347
Figure Lengend Snippet: (A) Chromatogram of liquid chromatography analysis with single nucleotides from Thanatos genomic DNA. (B) Mass spectra after isolation and tandem MS fragmentation of m/z 360.10 measured in positive ionization mode after dephosphorylation of nucleotides to nucleosides using alkaline phosphatase.
Article Snippet: Genomic phage DNA was digested using either a combined treatment of DNaseI and ExoI to create single nucleotides or a commercially available nucleoside digestion mix (NEB, Ipswich, USA).
Techniques: Liquid Chromatography, Isolation, De-Phosphorylation Assay